AmpC Disk Test for Detection of Plasmid-Mediated AmpC β-Lactamases in Enterobacteriaceae Lacking Chromosomal AmpC β-Lactamases

Plasmid typing and genetic context of AmpC β-lactamases in Enterobacteriaceae lacking inducible chromosomal ampC genes: findings from a Spanish hospital 1999-2007.

OBJECTIVES To gain insights into ampC transmission between bacterial strains. METHODS We examined the genetic context of 117 acquired ampC genes from 27119 Enterobacteriaceae collected between 1999 and 2007. Plasmid analysis was carried out by PCR-based replicon or relaxase typing, S1-PFGE and Southern hybridization. I-CeuI/PFGE was used for isolates not characterized by plasmid analysis. PCR...

AmpC β lactamases in Urinary Klebsiella pneumoniae Isolates: First Report of ACC Type AmpC β-lactamase Resistance in Iran

Background & Objective: The production of plasmid-mediated AmpC beta-lactamases (PMABLs) among urinary Klebsiella pneumoniae isolates causes a severe problem to the successful treatment of urinary tract infections (UTIs). This study was designed to evaluate antimicrobial resistance, the presence of AmpC beta-lactamase genes, and the genetic relatedness among K. pneumoniae strains separated from...

Kinetic Properties of Four Plasmid-Mediated AmpC -Lactamases

Kinetic Properties of Four Plasmid-Mediated AmpC -Lactamases Cédric Bauvois, Akiko Shimizu Ibuka, Almeida Celso, Jimena Alba, Yoshikazu Ishii, Jean-Marie Frère, and Moreno Galleni* Centre d’Ingénierie des Protéines, Université de Liège, Sart Tilman, Belgium; Department of Food and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan; and Department of Microbiology, ...

Detection of plasmid-mediated AmpC β-lactamase in Escherichia coli

Escherichia coli (E. coli) is a common opportunistic pathogen for nosocomial infection. The aim of the study was to examine the phenotype, genotype and epidemiology of plasmid-mediated AmpC β-lactamases in E. coli. In total, 96 clinical isolates of repeated E. coli were collected from different hospitals between August and October 2012. Using a cefoxitin disk diffusion method to identify the ph...

Alain Philippon , Guillaume Arlet and George A . Jacoby - Lactamases β Plasmid - Determined AmpC